Lab solution recipes
Trusted recipes for the reagents you reach for most — each one rescales every ingredient to the final volume you enter.
0.5 M EDTA (pH 8.0)
How to prepare a 0.5 M EDTA stock at pH 8.0 — the chelating workhorse behind TAE, TBE,…
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50× TAE Buffer
Make a 50× TAE (Tris-acetate-EDTA) stock for agarose gel electrophoresis, then dilute …
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1 M Tris-HCl Buffer
Prepare a 1 M Tris-HCl buffer stock at your chosen pH (7.4, 7.6, or 8.0). The amount of HCl …
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1× PBS (Phosphate-Buffered Saline)
Standard 1× phosphate-buffered saline at pH 7.4 for washing cells, diluting samples, a…
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10× TBE Buffer
A 10× TBE (Tris-borate-EDTA) stock for high-resolution agarose and polyacrylamide gels…
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5 M NaCl Stock Solution
A saturated-range 5 M sodium chloride stock for adjusting ionic strength and preparing buffe…
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70% Ethanol (from 95% or 100%)
Prepare 70% v/v ethanol — the standard surface and equipment disinfectant — by d…
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10% (w/v) SDS Solution
A 10% w/v sodium dodecyl sulfate stock for lysis buffers and SDS-PAGE. Quantities scale from…
Get recipe →These recipes are educational references, not validated SOPs. Always consult the Safety Data Sheet and your institution's protocols before handling chemicals.