PBS is the default isotonic buffer in cell biology. This is the standard Dulbecco-style formulation at pH 7.4. Quantities below are per litre of 1× PBS.
Scale this recipe. Recipe makes 1 L. Make instead:
Ingredient amounts below update automatically.
Ingredients
| Component | Amount | Note |
|---|---|---|
| NaCl | 8 g | 137 mM |
| KCl | 0.2 g | 2.7 mM |
| Na2HPO4 | 1.44 g | 10 mM |
| KH2PO4 | 0.24 g | 1.8 mM |
| Deionised water | 800 mL | to final volume |
Method
- Dissolve all salts in about 800 mL of deionised water with stirring.
- Adjust the pH to 7.4 with HCl or NaOH using a calibrated meter.
- Make up to the final volume with deionised water.
- Sterilise by autoclaving or filtration; store at room temperature.
Notes & safety
For a 10× PBS stock, multiply each salt by ten and dilute 1:10 before use; note the pH may shift slightly on dilution, so confirm it at 1×.
Consult the Safety Data Sheet for each reagent and follow your institution's protocols. Wear appropriate PPE.
Related guides: How to Make a Buffer Solution · How to Prepare a Solution of a …
Equipment for this prep
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